|Flip-flop method: A new T1-weighted flow-MRI for plants studies |
Auteur(s): Buy Simon, Le Floch Simon, Tang Ning, Sidiboulenouar Rahima, Zanca Michel, Cañadas P., Nativel Eric, Cardoso Maida, ALIBERT Éric, Dupont Guillaume, Ambard D., Maurel Christophe, Verdeil Jean-Luc, Bertin Nadia, Goze-Bac Christophe, Coillot Christophe
(Article) Publié: Plos One, vol. 13 p. (2018)
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The climate warming implies an increase of stress of plants (drought and torrential rainfall). The understanding of plant behavior, in this context, takes a major importance and sap flow measurement in plants remains a key issue for plant understanding. Magnetic Resonance Imaging (MRI) which is well known to be a powerful tool to access water quantity can be used to measure moving water. We describe a novel flow-MRI method which takes advantage of inflow slice sensitivity. The method involves the slice selectivity in the context of multi slice spin echo sequence. Two sequences such as a given slice is consecutively inflow and outflow sensitive are performed, offering the possiblility to perform slow flow sensitive imaging in a quite straigthforward way. The method potential is demonstrated by imaging both a slow flow measurement on a test bench (as low as 10 μm.s−1) and the Poiseuille’s profile of xylemian sap flow velocity in the xylematic tissues of a tomato plant stem.