- On the effect of local sample slope during modulus measurements by contact-resonance atomic force microscopy doi link

Auteur(s): Heinze K., Arnould O., Delenne J.-Y., Lullien-Pellerin V., Ramonda M., George M.

(Article) Publié: Ultramicroscopy, vol. 194 p.78 - 88 (2018)

Ref HAL: hal-01869770_v1
DOI: 10.1016/j.ultramic.2018.07.009
Exporter : BibTex | endNote

Contact-resonance atomic force microscopy (CR-AFM) is of great interest and very valuable for a deeper understanding of the mechanics of biological materials with moduli of at least a few GPa. However, sample surfaces can present a high topography range with significant slopes, where the local angle can be as large as ± 50°. The non-trivial correlation between surface slope and CR-frequency hinders a straightforward interpretation of CR-AFM indentation modulus measurements on such samples. We aim to demonstrate the significant influence of the surface slope on the CR-frequency that is caused by the local angle between sample surface and the AFM cantilever and present a practical method to correct the measurements. Based on existing analytical models of the effect of the AFM set-up's intrinsic cantilever tilt on CR-frequencies, we compute the non-linear variation of the first two (eigen)modes CR-frequency for a large range of surface angles. The computations are confirmed by CR-AFM experiments performed on a curved surface. Finally, the model is applied to directly correct contact modulus measurements on a durum wheat starch granule as an exemplary sample.